Tag Archives: Microscopy

If I have seen further than others it is because I have a bloody big microscope

Advanced microscopy has emerged as a powerful tool in microbiology. Here’s why. Continue reading

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Single-Cell Imaging of HIV-1 Proviruses

New works demonstrates that integrated HIV DNA localizes at the periphery of the nucleus, revealing important insights in the nuclear biology of HIV-1. Continue reading

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Detection of HIV-1 in Living Cells

In basic and applied HIV research, reliable detection of viral components is crucial to monitor progression of infection. While it is routine to detect structural viral proteins in vitro for diagnostic purposes, it was previously impossible to directly and dynamically … Continue reading

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Structure of Ebola virus [video]

Recently some of my final year virology students commented to me that it was a shame I was not still making regular MicrobiologyBytes podcasts. But as I said when I stopped posting weekly podcasts, I don’t feel that audio adds … Continue reading

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Native HIV-1 Budding Sites

The production of new HIV-1 particles is initiated at the plasma membrane where the virus polyprotein Gag assembles into a budding site, and proceeds through release of an immature virion which is subsequently transformed to the infectious virion by proteolytic … Continue reading

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Microbial nanoscopy

With its ability to observe single microbial cells at nanometre resolution, to monitor structural dynamics in response to environmental changes or drugs, and to detect and manipulate single-cell surface constituents, atomic force microscopy (AFM) provides new insight into the structure–function … Continue reading

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Giant microscope meets giant virus

Mimivirus appears most closely related structurally to large algal viruses such as PBCV-1 and other irridoviruses, though it possesses additional features not present in those viruses. The icosahedral capsid consists of 20 large triangular plates joined at their edges to … Continue reading

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Shedding light on the inner workings of the immune response

Leishmania donovani is a protozoan parasite that causes severe disease in humans with associated pathology in the spleen and liver. In experimental models of L. donovani infection, the hepatic response to infection is characterised by the presence of a focal … Continue reading

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HIV-1 Assembly and Release

Human immunodeficiency virus (HIV) particles are formed and released at the plasma membrane of the infected cell. Researchers analyzed the dynamics of HIV assembly and release making use of fluorescently labeled HIV structural proteins. They determined that assembly of the … Continue reading

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