Tag Archives: RNA

Over at Principles of Molecular Virology: RNAi – miRNA – siRNA – do you know the difference?

The Great RNA Confusion: RNAi – miRNA – siRNA – do you know the difference? Continue reading

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Tricks an IRES uses to enslave ribosomes

In eukaryotes, mRNAs are primarily translated through a cap-dependent mechanism whereby initiation factors recruit the 40S ribosomal subunit to a cap structure at the 5′ end of the mRNA. However, some viral and cellular messages initiate protein synthesis without a … Continue reading

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Self-amplifying RNA vaccines

Vaccines are a triumph of medicine and science – but is the pipeline running dry? What about all those viruses we have not been able to make effective vaccines against – HIV, RSV, Ebola, etc? DNA vaccines have generally proved … Continue reading

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Inhibition and avoidance of mRNA degradation by RNA viruses

The cellular mRNA decay machinery plays a major role in regulating the quality and quantity of gene expression in cells. This machinery involves multiple enzymes and pathways that converge to promote the exonucleolytic decay of mRNAs. The transcripts made by … Continue reading

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Rates of Virus Evolution Are Linked to Host Geography in Bats

Rapid evolution of RNA viruses is intimately linked to their success in overcoming the defenses of their hosts. Several studies have shown that rates of viral evolution can vary dramatically among distantly related viral families. Variability in the speed of … Continue reading

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First RNA virus-encoded miRNAs

Although the first miRNA was identified 18 years ago, it was only in 2001, with the development of technologies that allowed the efficient cDNA cloning and sequencing of small RNA species, that it became apparent that all multicellular eukaryotes encode … Continue reading

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The transcriptome of the adenovirus infected cell

By convention, the human adenovirus replication cycle is divided into two phases, an early and a late phase, which are separated by the onset of viral DNA replication. Based on temporal changes of the gene expression pattern as revealed by … Continue reading

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Negative strand RNA viruses – the state of the art

It was my priveledge to work with Brian Mahy many years ago. Brian has just retired as long-serving Editor of Virus Research, and his swansong is an excellent special issue on negative strand RNA viruses – an important read for … Continue reading

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Go FISH

The introduction of rRNA-targeted fluorescence in situ hybridization (FISH) using oligonucleotide probes for the cultivation-independent identification of microbes more than 20 years ago marked the beginning of a new era for environmental and medical microbiology. When integrated into the so-called … Continue reading

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